FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18587526
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA381930.20547651150561944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21850.023.6112041
TAGGACG97950.018.4361154
GTCCTAC129750.016.8292661
TAGACCG22150.015.9803274
GGACGTG104850.015.7340766
GTAGGAC136800.015.4808923
CTAGACG20850.015.2502573
TTGACGG28050.015.18375231
GGTACGA18600.015.1588618
TATGGCG111600.015.10368416
AGGACGT125150.015.0524145
CGGCTAT31500.015.044682535
CCGTACG16900.014.90789133
CTACACG28050.014.5439834
TACAGTG183050.014.060315
CTACAGT168050.014.0301214
CGTACGT16700.014.00924134
TGGCGAG122600.013.99449518
TACACCG25300.013.9906815
GTACGAG21100.013.93336619
Produced by FastQC (version 0.11.2)