FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18523749
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT400100.21599299364291752Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG71050.022.715229120
TCTCGGG54350.020.31174120
CGCCGTT29600.020.269228120
CGTCTAG22250.020.2266251
CGTATCC136950.020.064684120
TAGGACG95200.017.9003164
CGGTGGG66300.017.103193120
CCGACGG38750.016.72172120
CGTACGT17750.016.2274134
GACGAGG78650.016.01952120
CCGTACG19100.015.70960133
GTCCTAC133450.015.5128911
GGACGTG103750.015.3259616
CGTAATC20950.014.89275922
AGGACGT120900.014.7899985
TAGATCC170500.014.392221120
GTAGGAC134000.014.0162473
CTACGGT19050.013.85770513
TGCGACC26500.013.5841710
TGACGCC55200.013.477567120
Produced by FastQC (version 0.11.2)