FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18523749
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA376990.20351711740425765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG95800.018.4746254
CGTCTAG20300.018.323371
TCGTACG5003.7289283E-1016.7974497
GTCCTAC133300.016.3825281
GTAGGAC137250.015.8239643
CCGTACG17500.015.7684739
CGTACGT17050.015.48080110
GGTACGA19050.015.430387518
GGACGTG106250.015.1882726
CCGATAC19300.014.6086619
AGGACGT121400.014.4312645
ATACGCG23150.014.2538034
GTACGAG20650.014.23527219
TACGCGT23850.014.087695
TTGACGG24350.013.79532531
TACAGTG187150.013.7853815
TACACGG27300.013.6260265
CCCGATA28750.013.5628228
TGTTCCG40400.013.5145065
CGTCTAA20050.013.4650471
Produced by FastQC (version 0.11.2)