FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18515498
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT402250.2172504352839983Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG55250.021.500355120
CGCCGTT28950.020.309078120
CGGTGGG66900.018.922087120
CGTATCC145050.018.860788120
CCGACGG37650.018.80311120
TCGCCGG70850.018.798615120
CGTCTAG21350.018.2715741
TAGGACG93800.017.9145184
GTCCTAC131350.016.7228971
GGACGTG104650.016.2280626
TAGATCC178550.014.750861120
AGGACGT120350.014.6605815
TGACGCC57800.014.635379120
CGTACGT17700.014.24063834
CTACGGT17700.014.23648513
GGTCGGT37600.014.20105811
TACAGTG185750.013.82817655
GTAGGAC136700.013.6976663
GACGAGG78850.013.695678120
CCTACAG167950.013.5431513
Produced by FastQC (version 0.11.2)