FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18515498
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA379720.20508225055572366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21100.022.462081
TAGGACG91850.019.8562584
GTCCTAC133550.017.4747521
TTGACGG25000.017.0356731
GGACGTG101850.016.9642416
ACGTACG17350.016.25034937
CCCGATA29300.015.7649748
CGTACGG17650.015.63422138
TATGGCG107200.015.557343516
GTAGGAC134950.015.5152193
AGGACGT119700.015.3867625
GGTACGA17150.014.6916918
GGTCGGT37650.014.65861611
CTACAGT174500.014.4396554
TACAGTG186600.014.1463385
TGGCGAG118500.013.97260318
TTGACGA61550.013.93630560
ATGGCGA124050.013.78271117
CGTCTAA22250.013.7513551
GACGTGG66000.013.72470957
Produced by FastQC (version 0.11.2)