FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18491423
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT399470.21602988585572888Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21900.023.56871
TCTCGGG51450.021.4551120
CGCCGTT29650.021.447626120
CCGACGG37600.018.827482120
TAGGACG90350.018.7989544
CGTATCC150900.018.606073120
TCGCCGG72850.018.034855120
CGGTGGG63450.017.681051120
GTCCTAC132250.016.7006531
GGACGTG99450.016.5933046
TTGACGG27300.016.4872531
CGGCTAT33000.016.18500135
AGGACGT117400.015.3365415
GACGAGG80750.015.007451120
GGTCGGT39100.014.88377311
GGTACGA19050.014.80381918
CGTACGT19550.014.12084534
AGACCGT29300.013.9266376
ACGGCTA31550.013.8858734
TATGGCG107600.013.82962216
Produced by FastQC (version 0.11.2)