FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18491423
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA377870.2043487945735707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20200.020.1940671
TAGGACG99000.017.0874254
GGTACGA18100.016.57004218
GGACGTG109650.015.9732146
GTAGGAC138850.015.8124063
GTCCTAC128450.015.7851551
GTACGAG19500.015.07287319
GCGTACT21650.014.6836817
AGGACGT130250.014.599715
TTGACGG26300.014.59679831
TACACTG156600.014.0967435
GGTCGGT36450.013.988502511
CGTACGT15650.013.79817134
GCGAATT18450.013.65486619
TACAGTG181500.013.4518035
TACACCG23250.013.416625
ATTACGC16650.013.3306163
CTACAGT171350.013.19835954
CTAGACG21000.013.1401783
TGCGTAC23800.013.1052266
Produced by FastQC (version 0.11.2)