FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18445746
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT395650.21449390011116926Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG38600.020.983173120
TCGCCGG71600.020.194298120
TCTCGGG50600.020.038301120
CGCCGTT28300.019.504108120
CGTATCC153650.019.445606120
CGGTGGG62450.019.118128120
TAGGACG98000.018.309524
GTCCTAC132800.016.9459171
GGACGTG108150.016.589176
GACGAGG76300.015.411902120
AGACCGT28400.015.2122766
AGGACGT125600.014.8594475
GATGCGA22650.014.56935716
TACGCGT24000.014.5026925
GTAGGAC138150.014.4652253
TATGGCG103550.014.42763616
GCGAATT20600.014.27178619
GACCGTT23300.014.1628387
CGTCTAG22150.014.0883931
GGTCGGT39700.014.05436711
Produced by FastQC (version 0.11.2)