FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18445746
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA375810.20373803260654244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20850.019.2787441
TAGGACG96250.019.1966154
GGACGTG104400.017.1220726
GTCCTAC129200.016.4845031
GTAGGAC138300.016.3528633
CGTACGT15050.016.34241334
AGGACGT121700.015.8723035
CCGTACG16800.014.99715233
CGTAATC18750.014.39722622
CTAGACG20900.014.0645063
TATGGCG109200.014.06307316
TAGACGC41850.014.0477034
GCGTAAA19700.014.0088211
CGTCTAA20350.013.5613651
GACGTGG69500.013.5505027
TACACTG158600.013.4654695
TGGCGAG120500.013.391211518
TTGACGG27350.013.37954331
TACAGTG182150.013.3712365
CGGCTAT32250.013.20686635
Produced by FastQC (version 0.11.2)