FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18487464
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT395020.2136691111339013Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT22800.059.65731494
CGACGTA32300.047.58548492
TCGACGT35250.043.7962291
ACGCCGT31000.042.99945494
ACGTATC34950.041.83693394
CACCGTA34350.039.99384793
TGTCGGT29750.038.40527393
TAGATCG28150.037.93236588
GGTCGAC42600.036.24038789
TCGGTAG23350.035.82571489
TACGAGT20150.035.43910694
TGGTCGA43600.035.2541888
ATGTCGG35900.034.66771392
GTCGACG44950.034.49650290
GTGATCG28300.034.36525391
GTAGCCG45950.034.33768592
TAGTCGC23700.034.1481593
GACGTAT43550.033.7313893
GTAGTCG25450.032.86895492
TCACCGT46900.032.19205592
Produced by FastQC (version 0.11.2)