FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18487464
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA371670.20103893102915577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19350.022.6336361
TAGGACG95300.019.6414784
GTCCTAC135500.019.038891
CTACGGT20200.017.22441313
TCGTACG5254.0017767E-1117.14327299
GGACGTG105600.017.0418576
AGGACGT123000.016.242455
GTAGGAC139500.016.2133873
CGACTTG56650.016.00410864
TTGACGA61600.015.87301560
TACGGTG22000.015.81458714
GACGACT59550.015.61353862
TAGACGC44950.015.3490174
TCCTACA196750.015.2464132
CTAGACG21850.015.1014213
TGACGAC63650.015.07901561
TTGACGG27200.014.99707731
TACAGTG187150.014.9064965
CCTACAG169450.014.8347083
CGTACGT16400.014.63149310
Produced by FastQC (version 0.11.2)