FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21328559
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT685360.32133441363760207Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29200.029.1826341
TTGACGG30950.025.39648631
TCTCGGG55750.024.10833120
TCGCCGG70550.023.72855120
CTACGGT24400.021.88388613
CGTATCC129550.020.934616120
TACGGTG26950.019.5906214
CGGTGGG68900.019.420017120
TGACGGC40600.019.36012832
CGCCGTT25500.019.059383120
TCCGCAA28450.018.98117833
GACGGCT39300.018.62645733
TAGGACG73950.018.5018714
CCGACGG39000.018.308228120
CTAGACG31200.018.272083
ACGGCTA42650.017.44466234
CGGCTAT47600.017.39481535
CCTACGG31650.016.49186712
AGACCGT36450.016.1308736
CGCGTAT39900.015.9389347
Produced by FastQC (version 0.11.2)