FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21328559
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA646690.3032037935614872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27900.030.1012331
TACGGTG28600.026.21669414
CTACGGT28800.025.82635713
TTGACGG32700.023.29684331
TCCGCAA33300.021.97640633
CCTACGG34300.021.68525712
TAGACGC58750.021.0360184
CTAGACG37200.020.320383
ACGCACC35950.020.1912987
GACGCAC37550.019.970416
TAGGACG75750.019.7998354
CTGGTCG38250.019.4473699
GTCCGCA37150.019.3759732
ACGGTGA40300.019.35015515
CTACGCC68700.018.774422120
GCGAATT29250.018.66311319
GATGCGA29100.018.55286416
GACGGCT40900.018.03943333
CGGCTAT47450.017.95131535
TGACGGC42450.017.94597632
Produced by FastQC (version 0.11.2)