FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21214403
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT674700.3180386457257364Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT23750.026.527298120
CGTCTAG27100.026.35021
TCGCCGG70550.023.898829120
CCGACGG38050.023.653963120
CTACGGT21800.022.56767513
TCTCGGG56450.022.53405120
TTGACGG30900.022.1381531
CGTATCC131950.021.827257120
TACGGTG22450.021.6470214
CGGTGGG64000.018.375679120
GGTACGA24000.018.24921218
TAGGACG76850.018.0374164
ATCATCG30450.017.9328430
CTAGACG29250.017.8484133
TAGACCG27200.017.208064
TGACGGC41350.017.12385232
AGACCGT35050.016.9465686
GTACGAG26000.016.84554719
CGGCTAT48550.016.80740435
TCCGCAA29000.016.5533933
Produced by FastQC (version 0.11.2)