FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21214403
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA636950.30024413131022354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27100.028.1133841
TTGACGG32000.022.11984631
CTACGGT24950.021.39741513
TACGGTG25950.021.03516214
CGTACGT24000.020.24561534
CCGTACG24550.019.79172133
TAGGACG76150.019.5393734
CTACGCC72150.019.123262120
CTAGACG32000.018.7490333
TCCGCAA28550.018.69966933
GACGGCT39850.016.85930833
CGGCTAT46750.016.68088235
CCTACGG31700.016.65195712
TGACGGC42550.016.6353732
ACGCACC32650.016.5367957
TAGACGC58750.016.4417064
GGTACGA25300.016.3610218
GACGCAC34600.016.2985466
GTCCGCA33150.016.10484332
GTACGAG25750.016.07593319
Produced by FastQC (version 0.11.2)