FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21247134
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT669720.31520486480670756Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29450.026.2851561
CGCCGTT22650.023.311298120
TCGCCGG68650.023.161001120
TCTCGGG57050.021.770414120
TTGACGG32550.020.8356131
CTACGGT23550.020.38090513
CGTATCC136900.019.89777120
TACGGTG25150.019.56141714
CCGACGG37200.019.516163120
CGGTGGG65900.019.484102120
GGTACGA23100.018.44046218
CTAGACG29350.017.5831433
TCCGCAA29250.017.44052533
CGGCTAT46550.016.88521835
GTACGAG26050.016.81361219
TAGGACG73400.016.1873324
GACGGCT40050.016.03421833
TGACGGC42700.015.88288232
TATCGAA29100.015.87530748
CCTACGG31800.015.65940912
Produced by FastQC (version 0.11.2)