FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21247134
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA634620.2986849897026112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27000.029.106681
TTGACGG31100.022.95292331
GGTACGA23350.019.525218
CTACGCC66700.018.706345120
TACGGTG26000.018.4567314
CTACGGT26450.018.36954913
TGACGGC39600.018.17763932
TAGGACG75350.018.1541374
CGTACGT25400.017.94896934
CGACGAA28000.017.78447223
TCCGCAA27850.017.44661733
GTACGAG26750.017.26974119
CCGTACG26550.017.17119433
ATTCGAA30550.016.88646132
CGAATTG31450.016.7874222
TTGGACG30850.016.52846517
ATGTCGA30500.016.52145218
TGTCGAA33800.016.50764519
TACGAGG27700.016.4610620
TAGACGC57450.016.3958364
Produced by FastQC (version 0.11.2)