FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21268422
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT674290.31703809525690246Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG68800.024.76594120
CGTCTAG28500.024.6344851
CGTATCC130500.022.159575120
CCGACGG38100.021.416023120
TCTCGGG54950.021.181608120
CTACGGT22750.021.09826513
TTGACGG32600.020.98609431
CGCCGTT22200.020.809547120
CGGTGGG63400.020.251139120
CTAGACG27500.018.9840583
TACGGTG25650.018.946814
TGACGCC53450.018.52086120
CGTACGT24500.018.37042234
TCCGCAA28600.017.83599333
CCGTACG25400.017.24779333
TAGACGC53750.017.0805234
TAGGACG77250.016.8558044
CCTACGG30350.016.6057612
TGACGGC42650.016.46308532
TGCTCGA32150.016.42194410
Produced by FastQC (version 0.11.2)