FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21268422
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA635990.2990301772270646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25450.025.6945111
CTACGGT27150.022.3157413
TACGGTG26300.021.89740214
GGTACGA25250.020.43225118
CGTACGT20700.020.28443534
TCCGCAA28750.020.23807133
CCTACGG30450.019.70028312
GTACGAG27400.019.04775419
TTGACGG31050.018.9321431
CCGTACG22850.018.90085833
GTCCGCA32500.018.27204132
CTAGACG33300.018.196463
TAGGACG75850.018.0338754
TACGAGG30300.017.22474120
ACGGTGA35500.017.0675115
CGTCTAA29000.016.963581
TAGACGC58800.016.9371594
GACGCAC36350.016.8337176
GTCCTAC130650.016.4389931
CTACGCC69400.016.422472120
Produced by FastQC (version 0.11.2)