FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21146099
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT662250.3131783313792298Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27250.028.1898421
CCGACGG39050.025.1961120
TCGCCGG72450.024.511158120
TTGACGG30450.023.05998631
TCTCGGG55100.021.449894120
CGTATCC135850.021.374514120
CGCCGTT25050.020.357372120
CGTACGT25200.018.81403234
TAGGACG75500.018.7588864
CGGTGGG63200.018.321068120
GGTACGA24350.018.23375518
CTACGGT23250.018.06359113
CCGTACG26350.017.9931433
AACTGCG38800.017.9389237
TGCGACC34600.017.5128910
CGGCTAT45250.017.37297435
GACGGCT39150.017.32236333
TGACGGC40550.017.16831232
TACGGTG25500.016.4702514
ATCATCG34300.016.44728530
Produced by FastQC (version 0.11.2)