FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21146099
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA629610.29774286027886276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27200.030.6579861
TTGACGG33900.023.5338631
TACGGTG25950.021.2685514
CTACGGT26800.020.81547413
TGACGGC40150.019.87043232
CGTACGT25650.019.64437934
CCGTACG26550.019.4300633
CTAGACG32300.019.3165043
TCCGCAA28750.019.1950933
TAGGACG73750.018.465394
CGGCTAT48900.018.27779235
GGTACGA24650.018.25293718
GACGGCT41600.018.02423733
GACGCAC33300.018.0160946
CCTACGG31050.017.77449412
CTACGCC71650.017.583607120
GCGAATT28700.017.55951519
ACCGGTA30150.017.50820734
GTCCGCA31700.017.40883832
CCGTAAC29800.017.10975829
Produced by FastQC (version 0.11.2)