FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21109604
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT662600.31388556602009204Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25450.027.3565241
TCGCCGG70100.021.823664120
TCTCGGG52500.021.369177120
TTGACGG31100.020.64766331
CGTATCC143000.019.676249120
CGCCGTT28000.019.497944120
CCGACGG38850.019.148563120
TAGGACG72150.018.30044
GACGGCT38350.017.3701733
CGGTGGG66050.017.076172120
CCGTACG26850.016.53993833
TGACGGC40400.016.48876432
GCGAATT31800.016.41593219
TACGCGT39500.016.4093595
CGTACGT26450.016.33628334
ACGCGTA39750.016.1529546
TGACGCC55950.016.084097120
CGTCTTA30750.016.00047515
GTCCTAC130650.015.9867631
ATACGCG39800.015.9844264
Produced by FastQC (version 0.11.2)