FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21109604
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA632640.29969297387103994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24500.029.8727071
TACGGTG23500.022.71853614
CTACGGT23950.022.29146613
TTGACGG32050.020.58719431
CTACGCC71500.019.719374120
CCGTACG24450.019.62653533
CGTACGT23950.019.2849634
TCCGCAA26700.019.09590333
TAGGACG77100.018.9073524
CCTACGG28800.018.74549712
CTAGACG31000.017.9969983
GACGCAC32300.017.455956
TAGACGC54400.017.4235654
GACGGCT38300.017.0710533
GTCCGCA29950.017.02372632
GGTACGA24800.016.93089718
ACGGTGA33900.016.6333715
TGACGGC40850.016.44593232
AACGGAC26000.016.15015815
CGGCTAT46550.015.97844635
Produced by FastQC (version 0.11.2)