FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21089870
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT657020.3115334518420455Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27650.026.0437951
TCGCCGG68050.022.569923120
CTACGGT25050.022.51390313
CTAGACG28150.021.3176733
TACGGTG25900.021.31253614
TCCGCAA27550.020.47351633
CGCCGTT25300.020.393707120
TTGACGG31350.020.09731131
TCTCGGG56350.019.803286120
CCGACGG38850.019.612411120
CGTATCC146550.019.44581120
CGGTGGG68600.019.240522120
TAGGACG75050.018.6304514
CCTACGG31350.018.18022212
TAGACGC54150.017.7312624
GGTACGA25200.017.14322318
GTCCGCA31550.017.11706732
GCGAATT30100.016.94388419
ACGGTGA35650.016.49396115
TGACGGC38350.016.42896332
Produced by FastQC (version 0.11.2)