FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21089870
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA626030.29683919341371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25250.033.2645261
TTGACGG32350.024.84836631
TACGGTG24600.023.41038714
CTACGGT25050.022.75036413
GGTACGA26150.021.10405518
TGACGGC40900.019.94724532
CCTACGG30400.019.7331712
GACGGCT39600.019.3901933
CTACGCC75250.019.296436120
GTACGAG29000.019.23697919
CTAGACG32100.019.0620983
TCCGCAA29550.018.67659433
CGGCTAT46600.018.66605235
CGTACGT24450.018.64670434
TACGAGG31750.018.51575520
TAGGACG76350.018.3857944
CCGTACG25850.017.86888733
GACGCAC33600.017.8522956
TAGACGC54150.017.7254434
ACGGCTA46350.016.82522834
Produced by FastQC (version 0.11.2)