FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-M-ATAC-2-9_GCTACGCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-M-ATAC-2-9_GCTACGCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21177987
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTA616560.2911324858212445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25950.030.055621
TTGACGG33450.022.59669531
CTACGCC80000.019.651728120
CTACGGT23650.019.27755413
TAGGACG76750.019.1517944
TACGGTG23750.018.94335414
TGACGGC40350.018.4351932
GACGGCT39500.017.61689433
TCGTACG5555.456968E-1217.29730899
ATACGCG37950.016.7577574
TACGCGT38500.016.6741925
CTAGACG30950.016.6706853
TGCGACC32150.016.60659410
ACGCGTA38450.016.5376156
GGTCGGT52250.016.18826311
GGACGTG88250.015.8381546
AACTGCG39850.015.6558837
CGGCTAT50000.015.597321535
CTGGTCG40550.015.5335249
CCTACGG28300.015.47411912
Produced by FastQC (version 0.11.2)