FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29032768
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT794470.27364597133831675Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG38350.024.0972771
TCTCGGG79700.021.982925120
CGTATCC180100.021.921646120
TCGCCGG120400.020.382517120
CGGTGGG97200.019.56834120
CGCCGTT39200.019.43921120
GGTACGA42100.019.09643418
TAGGACG107700.018.9998994
CCGACGG68200.018.739422120
CTACGGT34250.018.39311613
TTGACGG48200.018.17480731
TACGGTG36600.017.04821214
CTAGACG40200.016.8680533
GTACGAG48000.016.74916319
GGACGTG121650.016.3740566
GTCCTAC195650.016.286481
TCCGCAA39800.015.98036833
TACGAGG52050.015.90698720
TGACGCC78500.015.134097120
CGAATTG47350.014.82525422
Produced by FastQC (version 0.11.2)