FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29032768
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA750770.25859401349537187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37000.030.8061
CTACGGT35450.022.33572213
CTAGACG39500.021.7194753
TTGACGG47600.021.54958531
TAGGACG113100.020.8998174
TACGGTG39350.020.27445614
TAGACGC79550.018.250894
GGACGTG128300.017.768266
TCCGCAA43100.017.53650533
CCTACGG45450.017.2895712
AACTGCG59100.016.9513177
CGGCTAT74350.016.86237735
GTAGGAC202300.016.7557143
GTCCTAC192250.016.6631981
GTCCGCA48050.016.35413732
TTGACGA78650.016.24504560
GACGGCT63900.015.95871233
GACGCAC53500.015.9229126
TCTTACG49350.015.55886244
TGACGGC67050.015.47736732
Produced by FastQC (version 0.11.2)