FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28969524
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT790700.2729420062269577Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG39750.026.116561
TCTCGGG75200.023.537695120
CGTATCC189800.020.706411120
CGGTGGG108250.019.566204120
CGCCGTT41350.018.86373120
TTGACGG50450.018.79228831
TCGCCGG120750.018.534525120
TAGGACG111800.018.517584
CTACGGT35500.018.25271213
TACGGTG36800.016.9557714
CTAGACG42300.016.456013
GTCCTAC191000.016.1486681
CCGACGG67250.016.059793120
GGACGTG125700.015.894346
TCCGCAA41600.015.7223333
GGTACGA38250.015.68566118
TAGACGC79750.015.650914
CGTGATT81650.015.43287628
GACGGCT62800.015.0966733
TAGATCC252100.014.899142120
Produced by FastQC (version 0.11.2)