FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28969524
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA744980.2571599036283786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG34750.030.210541
TTGACGG49500.020.11678731
TAGGACG113050.019.9539344
CTACGGT36350.018.97768413
CTAGACG40600.018.9145133
TACGGTG37400.018.28449614
TCCGCAA39900.017.2894333
GGTACGA42750.016.9800418
GTCCTAC196150.016.5762461
GGACGTG131200.016.5525516
GTACGAG46400.016.03300519
GCGAATT44700.015.97168219
GACGGCT60500.015.76512933
TAGACGC78000.015.6908624
TGACGGC66750.015.6370132
AACTGCG57700.015.5955667
GTAGGAC197850.015.586133
GATGCGA49300.015.57547516
TAGGGCG58550.015.47055131
CCGTAAC46150.015.46772729
Produced by FastQC (version 0.11.2)