FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28997821
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT774670.26714765912928423Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG39700.024.789171
TCTCGGG78600.024.50397120
CTACGGT32000.021.37358513
CGGTGGG100050.020.809725120
TCGCCGG116350.020.57598120
TAGGACG113200.020.0379834
CGTATCC191200.019.393427120
TACGGTG36300.019.17228514
CGCCGTT40900.018.630922120
TTGACGG51450.017.8475431
GGACGTG130300.017.1299276
CTAGACG41100.017.0825483
CCGACGG67250.016.505678120
TCCGCAA41450.016.3610833
TAGACCG42650.015.7582374
CCTACGG43850.015.7344212
GTCCTAC197150.015.7058531
TAATCGA35300.015.46743821
CGTACGT39250.015.44246334
GGTACGA40850.015.27446618
Produced by FastQC (version 0.11.2)