FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28997821
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA733290.25287762139093145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG36400.037.413521
TTGACGG52100.024.40953631
GACGGCT60400.020.95592933
TAGGACG114300.020.630034
CTACGGT36950.020.6173113
TGACGGC66750.019.50158532
CTAGACG41600.019.4712563
TACGGTG39600.019.23748214
TAGACGC77500.018.7355824
CGGCTAT74550.018.18556235
GGACGTG127950.017.6293056
TCCGCAA43350.017.4358433
ACGGCTA73550.017.04627634
CCTACGG45600.016.83789612
GACGCAC49450.016.49916
GTCCGCA47750.015.70355832
GTCCTAC192650.015.539451
GTAGGAC205750.015.2807623
GGTACGA39450.015.054646518
CTGGTCG67500.014.9306579
Produced by FastQC (version 0.11.2)