FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28919710
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT778210.2690932931208508Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37850.027.2694381
TCTCGGG74700.025.138912120
CGTATCC188400.022.35522120
CGCCGTT40450.021.803267120
TCGCCGG117400.021.412563120
TAGGACG107150.021.1685684
CGGTGGG99350.020.049011120
TTGACGG48700.019.59420231
CCGACGG70250.018.361786120
GGACGTG125650.016.8097026
GTCCTAC193650.016.6716421
GACGAGG144500.015.403833120
TAGACGC80800.015.2241954
TGACGGC66150.014.87896332
GACCGTT50000.014.7603597
TTAGACG48250.014.6756823
TAGATCC262250.014.618679120
AACTGCG58650.014.4249047
CTACGGT34150.014.40650613
GACGGCT63900.014.36975333
Produced by FastQC (version 0.11.2)