FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28919710
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA732720.2533635364946606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG36150.034.1863331
TTGACGG50450.023.30376831
TAGGACG114150.020.3918274
GGTACGA40300.018.4581618
GACGGCT62250.018.4044933
TGACGGC66500.017.76949532
GGACGTG128900.017.452656
GTACGAG43200.017.21889319
CTACGGT34950.016.99215713
GCGAATT45050.016.7781119
TACGGTG35450.016.752914
CGGCTAT73150.016.48211735
CGTACGT36050.016.47250634
CTGGTCG66650.016.4707349
ACGGCTA71350.016.14124334
ACGTACG40150.015.98614537
GTCCTAC186950.015.9807611
ATGCGAA49200.015.97268817
TAGACGC78350.015.9266584
CTAGACG40150.015.838743
Produced by FastQC (version 0.11.2)