FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28855510
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT772010.2675433565374516Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37000.027.5735931
TCTCGGG80050.026.231565120
CGGTGGG95250.021.541632120
CGCCGTT40700.021.22685120
CGTATCC199800.021.049414120
TCGCCGG112850.020.414896120
TAGGACG107500.020.3761944
TTGACGG48100.020.0876231
CTACGGT36050.018.63994213
CCGACGG65850.017.12851120
TACGGTG38050.016.8721314
GGACGTG125200.016.6316456
TCCGCAA41600.016.01317233
CTAGACG41900.015.8984633
TAGACGC80600.015.7848154
AGACCGT57500.015.3412086
TGACGGC69300.015.15488132
GTCCTAC195250.015.060911
CGTACGT40050.014.98436634
GACGGCT64400.014.91014333
Produced by FastQC (version 0.11.2)