FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28855510
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA731490.2535009778028529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37400.032.5632171
TTGACGG50250.022.56179831
TAGGACG109250.019.6039854
CTAGACG39450.019.0092893
GACGGCT61400.018.26920533
CTACGGT36300.018.1772113
TGACGGC65250.017.83480632
TACGGTG36200.017.7319414
CGGCTAT74700.017.02409235
TAGACGC78800.016.9014664
GCGTAAA39900.016.2387921
TAGACCG41700.016.1131174
CTGGTCG66650.016.1113979
GTCCTAC191100.016.1049561
TGGTCGG58650.015.95647210
GGTACGA41050.015.92927218
GGACGTG127350.015.9231756
ACGGCTA70650.015.79244834
TCCGCAA43300.015.37735133
TACGCGT59550.015.2122275
Produced by FastQC (version 0.11.2)