FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28823903
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT767100.2661332852806228Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG40550.027.086481
TCTCGGG74850.022.762972120
TTGACGG47500.021.35226631
CGTATCC207600.021.269285120
TCGCCGG115650.020.698063120
TAGGACG109500.020.1705154
CGCCGTT41550.020.069944120
CGGTGGG99050.019.987658120
CCGACGG64750.019.271965120
CTACGGT33500.018.44658313
CTAGACG39750.017.8171063
GGACGTG123800.017.35316
TACGGTG35800.017.26260614
GTCCTAC193550.016.342171
CCTACGG41550.016.17208512
TAGACGC82150.016.0730534
CGGCTAT72050.015.9921235
TCCGCAA40450.015.72672233
TGACGGC67100.015.47299732
GACGAGG148400.015.443008120
Produced by FastQC (version 0.11.2)