FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28823903
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA729790.2531891673379556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG36800.030.157951
TTGACGG49900.021.03623231
TAGGACG114200.020.4865174
GACGGCT57400.017.97409233
GGACGTG127400.017.702686
GGTACGA40100.017.6509818
CTACGGT37350.017.34508313
CTAGACG42150.017.07873
TAGACCG42050.016.8339354
TGACGGC62450.016.71272532
TACGGTG39000.016.61159914
TAGACGC79200.016.5877914
GTACGAG43150.016.54238519
GTAGGAC199250.016.468733
GTCCTAC182900.016.235641
CGGCTAT71450.015.69892135
TCCGCAA40850.015.41798833
CGTACGT39800.015.3726634
CGGTGTA45250.015.2442199
TAGGGCG58700.015.22574831
Produced by FastQC (version 0.11.2)