FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28785469
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT774790.2691601099151798Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37000.024.4909631
TCTCGGG74300.022.52853120
CGGTGGG98900.021.535229120
CGTATCC207200.021.39795120
TCGCCGG118300.021.249403120
CGCCGTT39850.020.625746120
TAGGACG106950.019.5267124
CCGACGG66450.018.599005120
CTACGGT32900.018.41840413
TTGACGG47700.017.86325531
GTCCTAC193400.017.128261
TACGGTG35150.016.8986314
GGACGTG122100.016.6105256
GGTCGGT85200.015.77339311
TGACGCC91850.015.741835120
CTAGACG40800.015.5910843
CGTACGT41250.015.41955334
TAGACGC78950.015.2782834
CCGTACG43650.014.84670733
TCCGCAA40450.014.68612833
Produced by FastQC (version 0.11.2)