FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28785469
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA735130.25538232501961317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37800.031.107271
TTGACGG49800.021.68233731
TAGGACG112600.020.8309364
CTACGGT34350.019.38517613
TACGGTG35450.018.95288514
CTAGACG40800.018.0848713
GTCCTAC186400.017.86261
GGACGTG132400.017.533016
TAGACGC80200.016.605394
CGGCTAT72900.016.21080835
TCCGCAA39600.016.20882433
CCTACGG43650.016.07931512
GACGGCT64200.016.07149733
GGTACGA42100.015.67355318
CGTACGT37850.015.53184834
GTAGGAC199350.015.4373083
TATCGAA43550.015.42855448
TGACGGC69350.015.39701332
GTCCGCA45350.015.21190132
TACAGTG249200.015.165565
Produced by FastQC (version 0.11.2)