FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28896796
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT762170.26375588490848606Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT34300.054.95810394
CGACGTA45750.047.59978592
TGTCGGT43750.043.55380293
ACGTATC49600.041.43538394
ACGCCGT44050.037.6955494
CACCGTA53200.036.45686393
TCGACGT60500.035.9950291
GGTCGAC62050.035.4257389
TAGATCG39200.033.50758488
TGGTCGA66350.033.13091388
GTCGGTG58700.032.57724494
TACGAGT28300.032.22288594
GTGATCG44000.031.7065591
GTGGACG59000.031.25840690
ATGTCGG66750.030.68748792
TGATCGC45050.030.36318892
CGCAGTA60800.030.3328791
TGGACGC62100.029.58829991
TCGGTAG32850.029.4178689
TGCCGAT47000.029.24996688
Produced by FastQC (version 0.11.2)