FastQCFastQC Report
Mon 11 Dec 2017
NOD-clean-F-ATAC-2-7_CTCTCTAC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNOD-clean-F-ATAC-2-7_CTCTCTAC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28896796
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA719250.24890302717297794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG38500.030.3865241
TAGGACG112300.021.6364214
TTGACGG51050.021.62156531
GACGGCT57750.018.48983833
CTACGGT34600.018.37785713
CTAGACG40100.018.2522263
GGACGTG132950.017.958176
GTCCTAC185050.017.312481
TGACGGC65500.017.03478832
TACGGTG38400.017.02760514
TAGACGC77350.016.4431764
CGGCTAT74600.016.32423235
TCCGCAA40650.016.2329433
CGTACGT35800.016.08619334
AACTGCG57850.016.073127
GTAGGAC203050.015.7775163
AGACCGT58200.015.0486386
CCGTACG38000.014.99702533
TACAGTG250700.014.9567015
CGTACGG43850.014.91304438
Produced by FastQC (version 0.11.2)