FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22492423
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG79200.018.4121024
CGTATCC166000.017.855879120
TCGCCGG93450.017.72116120
CGTCAGA47100.017.7099022
CGCCGTT34950.017.682858120
CGTCTAG29000.017.3821531
TCTCGGG76800.016.875433120
GCGTCAG52500.016.8027481
CCGACGG54100.016.636286120
GACGAGG108100.016.318642120
AGGACGT89650.015.9964835
CGGTGGG100050.015.352718120
GGTACGA24800.015.24110618
GTCCTAC134800.014.9134181
TAGATCC191800.014.390359120
TTGACGA50000.014.27915960
GGACGTG97650.014.19290356
CGTACGT22650.013.77396310
CGACTTG51700.013.69367764
GTAGGAC134900.013.6123073
Produced by FastQC (version 0.11.2)