FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22492423
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1283790.5707655418004544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG43800.021.36429811
CGTCTAG28350.020.9484161
CGTCAGA45450.019.2702791
TAGGACG82500.018.6162724
CTGACGC58250.016.47639512
AGGACGT92750.016.0413745
CTAGACG28950.015.5424463
CTACGGT24850.015.20723813
CGACTTG52900.015.10800764
TTGACGA54050.014.98198960
TAGACCG25850.014.853414
GTAGGAC141500.014.754673
GGACGTG103900.014.7235326
GGTACGA25000.014.63719718
TACGCGT32350.014.4651895
TGACGCC43900.014.34699613
GCGGTAT33300.014.231541
TTAGTCG22350.014.22489539
TGACGAC60800.013.91063461
GACGACT58250.013.90174362
Produced by FastQC (version 0.11.2)