FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22467700
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG76150.020.013918120
CGTATCC169600.019.776722120
CCGACGG51900.018.960283120
TCGCCGG100950.018.306812120
TAGGACG77000.018.002234
CGCCGTT37000.017.67637120
CGTCTAG30050.017.5728931
CGGTGGG97700.016.582026120
GACGAGG108450.015.878909120
AGGACGT88050.015.6048285
CGTCAGA44200.014.9339772
TAGATCC200200.014.865719120
GTCCTAC131200.014.8646461
GGACGTG96500.014.2999676
GCGTCAG51950.013.9767071
TACGCGT33250.013.3534325
GTAGGAC137950.012.7453263
TATGGCG96550.012.73890716
TACAGTG189950.012.6665385
TAATGCG26250.012.5729874
Produced by FastQC (version 0.11.2)