FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22467700
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1279050.5692839053396654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25400.022.2010481
CGTCAGA43850.021.478771
TCTGACG42350.020.67982511
TAGGACG79250.017.4118794
CGACGAA19850.016.62193323
CGTATCG16700.016.52299710
GTCCTAC131350.015.93951
GTAGGAC137800.015.7607323
CTGACGC58000.015.61695612
AGGACGT93150.014.4271075
CTAGACG28150.014.2795153
TACACGG35900.014.2049145
CGTACGT19450.014.1868410
CTACGGT24950.014.18498513
TTGACGA52050.014.06003960
TACGCGT31350.013.9700985
TACGGTG26350.013.65897514
GCGTCAG45800.013.6221981
TAGACCG27800.013.5960514
GACGCCT40600.013.5928114
Produced by FastQC (version 0.11.2)