FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22439156
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG96050.020.739305120
CGCCGTT34600.018.208187120
TAGGACG81850.018.1090894
CGTATCC172300.018.108046120
CGGTGGG101500.017.320288120
CCGACGG50850.017.109234120
CGTCTAG28150.017.0541441
TCTCGGG76550.015.8328705120
AGGACGT93550.015.5217465
TACGCGT33000.015.2733535
CGTCAGA46600.014.937932
GCGTCAG53500.014.8060261
GGTCGGT47600.014.36889511
GTCCTAC132600.014.1650841
ACGCGTA34700.014.00583656
GACGAGG110050.013.902843120
GTAGGAC135450.013.8670373
CTACGGT24800.013.78950213
TAGATCC214100.013.731973120
GGACGTG102950.013.5794796
Produced by FastQC (version 0.11.2)