FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22439156
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1268350.565239619529362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25700.024.9762571
CGTCAGA47450.022.3776131
TCTGACG43050.021.59720411
TAGGACG80350.018.6670364
CTGACGC57550.016.36421812
AGGACGT90000.015.7988735
GGTACGA21350.014.89238918
GTAGGAC138500.014.7715433
CGGCTAT44350.014.74341135
GTCCTAC132200.014.4755791
ACGCGTA33000.013.8159336
GGACGTG101750.013.796286
GTGTAGA132350.013.7339491
TACGCGT34050.013.5673135
CTGGTCG43000.013.113629
GCGTCAG49400.012.993721
GCGTAAT21850.012.9039641
GGTCGGT48400.012.8892311
GACGCCT43450.012.83902814
CTACGGT22900.012.83516713
Produced by FastQC (version 0.11.2)