FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22407301
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG98050.020.253576120
TAGGACG80750.018.7262614
CGTATCC167900.018.68841120
TCTCGGG74700.017.187572120
CCGACGG53950.016.903378120
CGTCTAG27200.016.7667311
AGGACGT90100.016.5831745
CGCCGTT35200.016.021624120
CGTCAGA46100.015.8804442
GGACGTG95700.015.424276
CGGTGGG97150.015.377222120
GACGAGG106050.015.04847120
GTCCTAC134100.015.0353741
GCGTCAG53050.014.7048871
TAGATCC214250.014.505419120
TACGCGT33850.014.0043025
GTAGGAC137250.013.1163393
ACGCGTA35350.012.9004726
GACGTGA61500.012.8786287
TATGGCG94200.012.86590116
Produced by FastQC (version 0.11.2)