FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22407301
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1262550.5634547418272285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27400.022.552511
TCTGACG45250.022.53668811
CGTCAGA44850.020.4661981
CTACGGT23750.018.94274113
CTAGACG29900.018.8615553
TAGGACG79950.018.8354534
CTGACGC58000.018.4093812
TACGGTG25100.017.92414914
TAGACGC32500.016.0604134
AGGACGT91400.016.0173855
GTAGGAC139800.015.0633233
GTCCTAC133400.015.0211
GGACGTG98100.014.9825556
TCGTACG5702.390152E-914.73505397
TGACGCC42700.014.32905413
GTGTAGA130850.014.3050411
TATGGCG98150.014.179116
CGTATCG17500.014.05418210
GACGCCT44300.013.9471214
CGTAATC25950.013.86978722
Produced by FastQC (version 0.11.2)